Sartorius VS-AVPQ102 Vivapure Kit for Fast Purification

Sartorius, VS-AVPQ102, Vivapure AdenoPACK 100 RT, Kit for Fast Purification

Includes:

• 2 x Sartobind Q 15 units with protective end caps
• 4 x Minisart plus in blister packs (Yellow)
• 2 x Tube set with one-way valve
• 1 x Benzonase Nuclease stock (12.5 U/µl) Store at -20°C
• 1 x 20 ml Elution Buffer
• 1 x 25 ml Loading Buffer
• 1 x 120 ml Washing Buffer
• 2 x 50 ml syringes
• 4 x 10 ml syringes
• 4 x Vivaspin 20 concentrators with 100 kDa membrane

Complete kit for fast purification of up to 1 x 10¹³ adenovirus type 5 particles from 500 ml cell culture. Contains 2 AdenoPACK 100 syringe filter units with Sartobind ion exchange membrane plus all buffers and further accessories. Fast purification of up to1 x 10¹³ viral particles Vivapure AdenoPACK 100 is optimally suited for adenovirus purification from up to 200 ml cell culture for in vitro transfection. This flexible kit contains two AdenoPACK 100 units, which can be either used in tandem for the purification of up to 200 ml cell culture supernatant or individually for purifying virus from up to 60 ml cell culture. The purification is pursued manually with a syringe optimally attached to a retort stand. However, for even more convenience, protocols are provided for optionally running the virus purification with a peristaltic pump or with an infusion pump, in additional to detailed instructions for a manual operation supplied with the Kit. The accessories needed for the operation with a pump are supplied as individual products.

Features:

• High-speed process: completed in 2 hours
• High virus recoveries
• Convenient, easy to use
• For processing 20-200 ml cell supernatants