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Sartorius, VN02H43ETO, Vivacon Centrifugal Concentrator 100000 MWCO
Vivacon 2 are centrifugal unit for fast DNA concentration and buffer exchange of 2 ml samples. They are also the product of choice for protein fractionation applications. The use of Hydrosart ultrafiltration membrane results in high recoveries from dilute samples. Complete recovery of the concentrate is guaranteed by a reverse spinning step. Vivacon 2 is optimized for the concentration / recovery and buffer exchange applications of genomic DNA and peptides for samples up to 2 ml initial volume. This are also the product of choice for peptide fractionation applications in this volume range. For optimal performance with highly diluted samples, e.g. forensic specimens, Vivacon 2 is equipped with patented Hydrosart regenerated cellulose membranes with extremely low unspecific binding properties and high flux. Treated with a dual-cycle ethylene oxide (ETO) gas process to ensure this are sterile to a safety assurance level of 106 and free of detectable genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg. High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-offs printed on individual devices. Vivacon 2 features a horizontal membrane design optimal for DNA fractionation (primer removal) as well as peptide fractionation. The possibility of a reverse spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.
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